MecA/ClpC/ClpP AAA⁺ protease

Unit / Facility

Cryo-EM Swedish Nat. Facility (Stockholm)

System / Target

Central AAA⁺ protease MecA/ClpC/ClpP from S. aureus; near-atomic cryo-EM structure, integrated with AF modelling and functional assays.

Organism / Source

Staphylococcus aureus

Approx. Data Collection Years

~2022–2025

Access / Reuse Status

Public

Main Public Repository / Storage

PDB + EMDB + EMPIAR + publication supplementary data + Figshare / preprint assets + SciLifeLab Data Repository

Cryo-EM MS / Proteomics Biophysics (ITC/SPR/MST/DSF etc.) Integrative / computational modelling (non-MD)

PDB: 9GI1 (with EMD-51367; ClpC/ClpP body), 9GOQ (with EMD-51498; MecA crown), 9R2S (with EMD-53538; ClpP body), 9RAI (with EMD-53879; composite whole complex)
EMDB: EMD-51367, EMD-51498, EMD-53538, EMD-53879
EMPIAR: EMPIAR-12887 aligned micrographs and raw movie frames

Publication supplementary: Supplementary Data 1 (XLSX) source data for figures, Supplementary Video 1 (MP4) 3D variability / motions (MecA crown, ClpC/ClpP and ClpP bodies), Supplementary Information (PDF) figures, tables, extended methods

bioRxiv / Figshare (preprint): Preprint DOI AlphaFold predictions and ChimeraX session (MecA–ClpC AF models) linked from the paper
SciLifeLab Data Repository (Figshare): SciLifeLab Figshare record ChimeraX session and related assets for this publication
SciLifeLab Publication: SciLifeLab Publication

PDB-IHM: 9A66 B. subtilis integrative ClpC–ClpP model (XL-MS + deep learning); not the S. aureus MecA/ClpC/ClpP cryo-EM study
PRIDE: PXD035508 crosslinking-MS for PDB-IHM 9A66 (adjacent to this portal record, not the same dataset)

Publication: The EMBO Journal (2025) resting-state and peptide-bound ClpC/ClpP; distinct from the MecA/ClpC/ClpP cryo-EM study above
PDB: 9QCL, 9QRW, 9QQR
EMDB: EMD-53014, EMD-53324, EMD-53312