{
  "id": "ISB-D-0005",
  "portal_index": 5,
  "title": "FusB-mediated rescue of EF-G from fusidic acid",
  "portal_description": "Ribosome-bound EF-G stalled by fusidic acid and rescued by FusB; time-resolved cryo-EM \"movie\" combined with kinetics and MS quantification.",
  "portal_landing_page_url": "https://wpotrzebowski.github.io/isb_datasets/datasets/dataset-5.html",
  "unit_facility": "Ribosome/antibiotic-resistance structural biology (Uppsala-linked)",
  "system_target": "Ribosome-bound EF-G stalled by fusidic acid and rescued by FusB; time-resolved cryo-EM \"movie\" combined with kinetics and MS quantification.",
  "organism_source": "Bacterial 70S ribosome, EF-G, FusB",
  "approx_data_collection_years": "~2022–2024",
  "access": {
    "status": "public",
    "license": null,
    "license_url": null
  },
  "main_public_repository_storage": "PDB + EMDB + EMPIAR + proteomics repository",
  "techniques": [
    {
      "code": "cryo_em",
      "label": "Cryo-EM"
    },
    {
      "code": "ms_proteomics",
      "label": "MS / Proteomics"
    },
    {
      "code": "biophysics",
      "label": "Biophysics (ITC/SPR/MST/DSF etc.)"
    },
    {
      "code": "integrative_modelling",
      "label": "Integrative / computational modelling (non-MD)"
    }
  ],
  "repository_records": [
    {
      "kind": "PDB",
      "accession": "9GHA",
      "url": "https://www.rcsb.org/structure/9GHA",
      "relation": "primary_structure"
    },
    {
      "kind": "PDB",
      "accession": "9GHH",
      "url": "https://www.rcsb.org/structure/9GHH",
      "relation": "primary_structure"
    },
    {
      "kind": "EMDB",
      "accession": "EMD-51350",
      "url": "https://www.ebi.ac.uk/emdb/EMD-51350",
      "relation": "primary_map",
      "derived_from": "PDB:9GHA"
    },
    {
      "kind": "EMDB",
      "accession": "EMD-51357",
      "url": "https://www.ebi.ac.uk/emdb/EMD-51357",
      "relation": "primary_map",
      "derived_from": "PDB:9GHH"
    },
    {
      "kind": "EMPIAR",
      "accession": "EMPIAR-12731",
      "url": "https://www.ebi.ac.uk/empiar/EMPIAR-12731",
      "relation": "raw_data",
      "note": "Portal describes this as the raw cryo-EM movie."
    },
    {
      "kind": "MS repository",
      "accession": null,
      "url": null,
      "relation": "proteomics_dataset",
      "note": "Portal indicates an MS dataset exists but ID was not yet added (PRIDE/ProteomeXchange or similar)."
    }
  ],
  "publications": [
    {
      "type": "journal_article",
      "venue": "Nature Communications (2025)",
      "doi": "10.1038/s41467-025-58902-3",
      "url": "https://doi.org/10.1038/s41467-025-58902-3",
      "relation": "primary_publication",
      "title": "Structural mechanism of FusB-mediated rescue from fusidic acid inhibition of protein synthesis"
    }
  ],
  "keywords": [
    "FusB",
    "EF-G",
    "fusidic acid",
    "ribosome",
    "antibiotic resistance",
    "time-resolved cryo-EM"
  ]
}