{
  "id": "ISB-D-0001",
  "portal_index": 1,
  "title": "GLIC lipid-binding & gating",
  "portal_description": "Pentameric ligand-gated ion channel GLIC; cryo-EM structures in nanodiscs, lipid binding analysis, integrated with MD and earlier X-ray/SANS.",
  "portal_landing_page_url": "https://wpotrzebowski.github.io/isb_datasets/datasets/dataset-1.html",
  "unit_facility": "Cryo-EM Swedish Nat. Facility (Stockholm) / ISB",
  "system_target": "Pentameric ligand-gated ion channel GLIC; cryo-EM structures in nanodiscs, lipid binding analysis, integrated with MD and earlier X-ray/SANS.",
  "organism_source": "Gloeobacter violaceus",
  "approx_data_collection_years": "~2019–2023",
  "access": {
    "status": "public",
    "license": "CC BY 4.0",
    "license_url": "https://creativecommons.org/licenses/by/4.0/"
  },
  "main_public_repository_storage": "PDB + EMDB + SASBDB + SciLifeLab Data Repository",
  "techniques": [
    {
      "code": "cryo_em",
      "label": "Cryo-EM"
    },
    {
      "code": "xray_crystallography",
      "label": "X-ray crystallography"
    },
    {
      "code": "sans",
      "label": "SANS"
    },
    {
      "code": "biophysics",
      "label": "Biophysics (ITC/SPR/MST/DSF etc.)"
    },
    {
      "code": "md_simulation",
      "label": "MD / other simulations"
    },
    {
      "code": "integrative_modelling",
      "label": "Integrative / computational modelling (non-MD)"
    }
  ],
  "repository_records": [
    {
      "kind": "PDB",
      "accession": "8ATG",
      "url": "https://www.rcsb.org/structure/8ATG",
      "relation": "primary_structure"
    },
    {
      "kind": "PDB",
      "accession": "4NPQ",
      "url": "https://www.rcsb.org/structure/4NPQ",
      "relation": "related_structure",
      "note": "Mentioned on portal page as an older X-ray structure example."
    },
    {
      "kind": "EMDB",
      "accession": "EMD-15649",
      "url": "https://www.ebi.ac.uk/emdb/EMD-15649",
      "relation": "primary_map",
      "derived_from": "PDB:8ATG"
    },
    {
      "kind": "SASBDB",
      "accession": "SASDL43",
      "url": "https://www.sasbdb.org/data/SASDL43/",
      "relation": "saxs_sans_profile"
    },
    {
      "kind": "SASBDB",
      "accession": "SASDL53",
      "url": "https://www.sasbdb.org/data/SASDL53/",
      "relation": "saxs_sans_profile"
    },
    {
      "kind": "SASBDB",
      "accession": "SASDL63",
      "url": "https://www.sasbdb.org/data/SASDL63/",
      "relation": "saxs_sans_profile"
    },
    {
      "kind": "SciLifeLab Data Repository",
      "doi": "10.17044/SCILIFELAB.21908892",
      "url": "https://doi.org/10.17044/SCILIFELAB.21908892",
      "relation": "supplementary_dataset"
    },
    {
      "kind": "Researchdata.se catalogue",
      "url": "https://researchdata.se/en/catalogue/dataset/doi-10-17044-scilifelab-21908892",
      "relation": "catalogue_record"
    }
  ],
  "publications": [
    {
      "type": "journal_article",
      "title": "Discovery of lipid binding sites in a ligand-gated ion channel by integrating simulations and cryo-EM",
      "venue": "eLife (2024)",
      "doi": "10.7554/eLife.86016",
      "url": "https://doi.org/10.7554/eLife.86016",
      "relation": "primary_publication"
    },
    {
      "type": "preprint",
      "venue": "bioRxiv",
      "doi": "10.1101/2023.01.06.523006v1",
      "url": "https://doi.org/10.1101/2023.01.06.523006v1",
      "relation": "preprint"
    }
  ],
  "keywords": [
    "GLIC",
    "pentameric ligand-gated ion channel",
    "lipid binding",
    "nanodiscs",
    "integrative structural biology"
  ]
}